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Methylation of CpG dinucleotides results in symmetrically methylated CpG sites (i.e., methylation of the CpG sequence on both strands of DNA) (as seen in Fig
Expression and purification of recombinant APEX2 APEX2 was expressed from a plasmid (72558 pTRC-APEX2, Addgene) in Escherichia coli BL21(DE3) (EC0114, Thermo Fisher Scientific), as described previously 23
In addition to its relation to greying hair, a high level of homocysteine can mean an increased risk of cardiovascular disease
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For instance, Whitehouse et al
