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Tissues were then incubated in 10, 20, and 30% sucrose in 1 PBS, embedded in OCT compound (Tissue-Tek, Sakura Finetek, 4583), snap frozen on dry ice, and then stored frozen at 80C
(2012) 7:e47534
Dithiolethiones: a privileged pharmacophore for anticancer therapy and chemoprevention
2023;116 doi: 10.1016/j.intimp.2023.109762
Unlike many other vitamins, B12 is stored in the liver in substantial quantities, meaning deficiency typically develops gradually over months to years as these reserves become depleted
