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The plate containing the frozen cells were lyzed with Pierce RIPA Lysis and Extraction Buffer (Thermo Fischer Scientific) supplemented with 1 cOmplete Protease Inhibitor Cocktail (Roche) (220 L/well) for 10 min on ice on a tilting board
Excessive pressure pushes the needle deeper, potentially into muscle
[DOI] [PubMed] [Google Scholar] 65.Schulthess J, Pandey S, Capitani M, Rue-Albrecht KC, Arnold I, Franchini F, et al
About 95% of cases go away by 6 months of age
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