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In combination with histone modifications, DNA methylation has a fundamental role in modulating gene expression in germ cells by inhibiting the binding of transcription factors to enhancers or by recruiting the binding of proteins that facilitate the deacetylation or methylation of histones, thereby stabilizing the nucleosomes (142)

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The binary mobile phase composition and gradient elution program are detailed in the referenced publication
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10.1016/j.toxlet.2022.08.007 Toxicol Lett
