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We do not recommend splitting cells for more than 20-30 passages
10.1128/mcb.06120-11 54 FaresJ.FaresM

The above justification is explainable considering that selenoaminoacids are well documented for metabolic assimilation into bioavailable selenium [91]

The hypoxic niche in TME can accommodate cancer stem cells, which participate in drug resistance (195)

The IC 50 values for the ripe fruit pulp, ripe fruit seeds, unripe fruit pulp, and unripe fruit seeds were 27.78, 89.1, 77.33, and 75.2 g/mL for HepG2 cells, respectively