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Therefore, the production of highly active RPs is also an important optimization aim, which can be achieved by strengthening or supplementing PTMs, increasing proteolysis and overexpressing suitable molecular chaperones [26]

21 Principle: DTNB reacts with the sulfhydryl group of glutathione to produce yellow-colored 5-thio-2-nitrobenzoic acid, which has a maximum absorption peak at a wavelength of 412 nm, while DTNB has almost no absorption peak above 400 nm

Precipitation can also occur if incompatible substances contact the solution

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