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Description
Regular sterile water contains no such preservative, so once it's punctured it should be used immediately and discarded
Water down the wall, gentle swirl, refrigerate

Buffer selection and matrix effects should be validated for each method
Once a peptide is reconstituted, it is subject to: Hydrolysis Oxidation Structural changes over time These processes occur regardless of the solvent used

Aim for 1.2-1.6 grams of protein per kilogram of body weight during recovery
