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The resultant nuclei suspension was FACS sorted using 7-Aminoactinomycin D (7-AAD) dye as a singlet discriminator on a FACS Jazz cell sorter (BD Biosciences)

PMID: 32632640

In addition, low IGF-1 subjects in a study were found to possess up-regulated fatty acid metabolism along with down-regulated GLUT-1 gene (in charge of glucose uptake in erythrocytes, brain endothelial cells, eye, peripheral nerve and also responsible for materno-placental glucose transfer) [210]

Oxidative stress arises when excess ROS disrupts cellular balance, overwhelming the antioxidant defenses [151]

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