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Specific manifestations included increased malondialdehyde levels (reflecting lipid peroxidation), increased 8-hydroxydeoxyguanosine (indicating DNA oxidative damage) and decreased glutathione peroxidase activity and superoxide dismutase expression
Finally, the purity of the fractions was confirmed by Western blot analysis using cytoplasmic marker protein GAPDH and nuclear marker protein Histone H3(HH3)

11 , e1005110 (2015)

Dietary Reference Intakes for Vitamin C, Vitamin E, Selenium, and Carotenoids (National Academies Press, 2000)
In its developed form, the lesions appear as painful nodules with a tendency to scarring and abscess formation
