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The isobole can be derived using Eq

Histopathological findings further corroborate the biochemical results, with the MSG- and LPS-treated groups exhibiting degeneration of seminiferous tubules, interstitial oedema, and a reduction in germ cell layers

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Soil acidification is caused by either natural or anthropogenic processes that release hydrogen (H + ) ions into the soil [13, 14], including acid rain, weathering processes, organic matter (OM) decomposition, removal of cations through biomass of harvested material, imbalances in the carbon (C), nitrogen (N) and sulfur (S) cycles, and deposition of acidifying gases such as hydrochloric acid (HCl), nitric acid (HNO 3 ), ammonia (NH 3 ) and sulfur dioxide (SO 2 ), and sustained, continuous/annual application of acidifying nitrogenous fertilizers such as elemental sulfur (S), urea, ammonium nitrate, and ammonium sulphate [9, 15,16,17,18,19,20]