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Upon light excitation, flavins can undergo photoreduction through interactions with nearby amino acid residues, yielding highly reactive one-electron and two-electron reduced species 32,36,41,54

[DOI] [PubMed] [Google Scholar] 76.Wang R., Zhang L., Liao R., Li Q., Pi R., Yang X

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Despite the high catalytic activity and specificity of ALP, the moderate sensitivity of enzyme-linked immunoassays may restrict practical applications for determining low-abundance targets [37,41]