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The sample was injected into a Thermo Accela Ultra-High Performance Liquid Chromatography system coupled to a Thermo TSQ Quantum Tandem Mass Spectrometer (Thermo Electron, San Jose, California, USA)

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The implications of NNMT inhibition extend far beyond simple cofactor availability, influencing fundamental cellular processes including mitochondrial biogenesis, oxidative stress management, and epigenetic regulation

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Transcription and gene-expression studies: Measuring changes in ERR-regulated gene markers using reporter assays, qPCR panels, RNA sequencing, or protein-expression workflows