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Clinical studies have shown that vitamin D supplementation may lower circulating levels of pro-inflammatory cytokines, potentially mitigating neuroinflammation and improving social communication and language function in individuals with ASD ( 4.4 Trace elements and nutritional supplementation Children with ASD commonly exhibit elevated oxidative stress, characterized by a reduced glutathione-to-oxidized glutathione (GSH/GSSG) ratio and increased levels of malondialdehyde (MDA), a marker of lipid peroxidation (Efe et al., 2021)

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Ethics statement All surgical procedures and experimental manipulations were approved by the Committee of Ethics on Animal Experimentation in the Faculty on Medicine, our university (A28-251-0)

5.1 The related antagonists of Piezo1 Originally isolated from the venom of tarantulas, GsMTx4 is an amphipathic peptide with six cysteine residues and is the only known antagonist that specifically targets cationic mechanosensitive channels (MSCs) (Bosmans and Swartz, 2010