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Consistently, when cells were subjected to cystine starvation in the presence of 6-diazo-5-oxo-L-norleucine (DON, a selective GGT inhibitor), there was a markedly higher accumulation of extracellular GSH (Fig

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The modified protocol significantly improves the use of Ellman's reagent (DTNB) to measure GPx activity without needing protein precipitation