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After collecting inguinal subcutaneous adipose tissue from the mice, the tissue was incubated with collagenase D (Roche, 11088858001) at a concentration of 1.5 U/ml (with 10 mM CaCl 2 ) at 37 C for 45 min

Furthermore, bilateral renal-clamp studies were conducted to identify the effect of siRNA on the preservation of kidney function

Hallmarks of ferroptosis Morphological features Although an initial study indicated that ferroptosis is morphologically, biochemically and genetically distinct from apoptosis, necrosis and autophagy, 8 most investigators concur that cells undergoing ferroptosis usually show necrosis-like morphological changes

ScheijenJLJMHanssenNMJvan GreevenbroekMMVan der KallenCJFeskensEJMStehouwerCDAet al

HSP is a highly conserved family of stress-inducible proteins, essential for cellular homeostasis, protecting against a variety of stress stimulus [83, 84], injury, and death and modulating the early inflammatory response to muscle injury [30, 31]