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Cell culture Swiss 3T3 fibroblasts, obtained from American Type Culture Collection (ATCC CCL 92), were maintained at 37 in Dulbecco's modified Eagle's medium supplemented with 25 mM HEPES (pH 7.4), 10% (v/v) fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin

Further studies of mice engineered for CHAC and OPLAH that are involved in 5-OP metabolism may provide answers to this issue

Secondly, due to the ability to suppress all functions of a given protein, as well as undruggable proteins (e.g., transcription factors or scaffold proteins), since PROTAC does not need to bind to a specific site on the target protein 22

doi: 10.1111/acel.12219
