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coli BL21 (DE3) competent cells (Trans, CD601-02)
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doi: 10.3904/kjim.2021.458 117 PattapulavarV.RamanujamS.KiniB.ChristopherJ
Lymphocytes released from the spleen were fused to mouse myeloma P3X cells, and hybridoma clones were screened by immunostaining as Ishikawa (positive) and A431 (negative) cells

For all brain scans, the slices were positioned to run parallel to a line that joins the most infero-anterior and infero-posterior parts of the corpus callosum (ACPC line), covering the entire brain from the skull base to the vertex, with careful repositioning during the follow-up scan
