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The protein content of the resulting lysates was then quantified using the BCA Assay Kit (Pierce) and analysed on an absorbance plate reader (Tecan)

It is pivotal in transporting amino acids, bolstering the immune systems defenses, and safeguarding cells from oxidative damage

Because these pathways influence food intake, fat metabolism, and glucose regulation, amylin analogues such as cagrilintide are commonly used in biochemical assays, receptor-binding studies, and metabolic signaling research [4][5]

All these studies reinforce the important role played by tight junction proteins and highlight the central role of epithelial barrier function in the pathogenesis of IBD