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The metabolic benefits extend to cellular energy regulation, where NNMT inhibition increases intracellular NAD+ levels by 1.2-1.6 fold

Jul 2011;141(7):1355-61

however, these therapies are associated with numerous adverse reactions

The surnatants were transferred to mass spectrometry vials and directly analyzed using the UPLC 1290 (Agilent Technologies) coupled to the TripleTOF 5600+ mass spectrometer (SCIEX)
Chromatographic and electrophoretic methods for the analysis of biomarkers of oxidative damage to macromolecules (DNA, lipids, and proteins)