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Real-time qPCR was performed using an Applied Biosystems QuantStudio 12 K Flex real time PCR system (Thermo Fisher Scientific, San Jose, CA, USA) with THUNDERBIRD SYBR qPCR Mix (Toyobo), 6 pmol of primers, and aliquots of cDNA

In this process, melatonins involvement in the establishment of root architecture under adverse environmental stresses is also one of the important pathways to improve plant resistance

doi: 10.1080/10286020.2014.939584

A neighbor-joining method (Figure S1A

The absence of effects on IGF-1, glucose metabolism, and tissue growth means AOD-9604 avoids many common growth hormone side effects including edema, joint pain, glucose elevation, and concerns about cell proliferation