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The acquisition of viable autologous IVD cells is also problematic: harvesting cells from healthy discs risks iatrogenic damage, while cells derived from degenerated discs often exhibit reduced viability and functionality

[Google Scholar] 121.Ebeling A.W., Laur D.R
Curcumin's anti-inflammatory and antioxidant properties can help tackle these issues

2040 ug of total protein was separated on a 412% Bis-Tris gradient gel (Bio-Rad) by SDS-PAGE and then transferred to nitrocellulose membrane (Bio-Rad)

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