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The 2 Ct method was employed to determine the fold change in the RNA expression of one sample compared to the calibration sample
But those changes are typically gastric and appetite-related, driven by the GLP-1 component
doi: 10.1016/j.trsl.2007.10.003
Tissue Cell 82:102103 Hassanein EHM, Mohamed WR, Hussein RM, Arafa EA (2023b) Edaravone alleviates methotrexate-induced testicular injury in rats: implications on inflammation, steroidogenesis, and Akt/p53 signaling
Flow cytometry was performed on a BD FACSCalibur flow cytometer, and data analysis was performed with the accompanying software
