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The cells were washed twice with PBS, trypsinized, and then stained with propidium iodide(1 mg/mL)

We next established an acute zinc-overload environment through in situ pancreatic injection of ZnPTO in RIP-Cre

Icardi G, Orsi A, Ceravolo A, Ansaldi F

20 Primarily, NADPH provides reducing equivalents for fatty acid synthase (FASN), the main rate-limiting enzyme, to synthesize fatty acids with acetyl-CoA serving as a primer and malonyl-CoA as a two-carbon donor, 21,22 and provides the needed electrons for ironsulfur (Fe/S) protein assembly that participate in non-essential amino acid biosynthesis and lipoic acid synthesis, tRNA modification, DNA replication and repair, and telomere maintenance