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Results and discussion Synthesis of PEI-Coated/CRISPR plasmid nanoparticles complexes The DNA/PEI complexes are capable of escaping from endosomes or preventing endo-lysosome formation by the proton sponge effect 26

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10.1038/npp.2008.220 31 IrvineE
It combines these chemicals together with an ethynylation and then a Wittig reaction
