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Description
Answer: It varies from product to product
The 5 mg vial + 2 mL gives 2.5 mg/mL, so a 2 mg start is a large, easy-to-measure 0.80 mL (80 units) convenient early on, though larger doses later exceed one barrel

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Following 3 10min washes with 1ml 0,2% TBS-Gelatin and 1 wash with regular TBS the slides were mounted using soft set mounting medium with DAPI (Vectashield) and sealed with nail polish
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