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column and autosampler temperatures were 25 C and 4 C, respectively

It depends on individual factors such as metabolism, overall health, and the severity of B12 deficiency

Those three questions separate a grounded protocol from a hopeful one

The samples were centrifuged at 8000 rpm for 5 min, washed twice with 30 mL sterile saline, and resuspended in M9 minimal medium supplemented with 10 mM ammonium acetate, 1 mM MgSO 4 , and 100 M CaCl 2
Importantly, methylated esters of TCA intermediates, such as DMF, are cell permeable and can modify the activity of this pathway by increasing the level of metabolic intermediates' proximate to fumarate
