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The His6-SUMO tag was cleaved by ULP1 treatment, and the tag-free protein was fractioned with Ni-NTA column and Superdex 75size exclusion column (GE Healthcare) with 25 mM HEPES (pH 8.0), 300 mM NaCl, and 1% glycerol

Xie H, Yang DH, Yao H, Bai G, Zhang YH, Xiao BG (2016) iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco ( Nicotiana tabacum ) in response to drought stress

Following the sacrifice of the rats with 0.8 ml kg 1 body weight of ketamine hydrochloride, the cauda epididymides were rapidly removed and minced in the modified BiggersWhittenWhittingham (BWW) capacitation medium, whose components have been previously described [17, 25]

Wound Healing and Repair: GHK-Cu accelerates tissue repair by boosting collagen synthesis and promoting the formation of new blood vessels that deliver oxygen and nutrients to damaged areas