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After the addition of DTT (15 l, 31 mg ml 1 stock in ddH 2 O) to quench excess IAA, samples were shaken at 37 C for further 30 min (850 rpm, Thermomixer, Eppendorf)

10.1523/jneurosci.1007-05.2005 86 MorganC

Int J Clin Pharmacol Therapeut

Although inexpensive and easy to carry out, this method is only able to detect cases with 20% or less of mean normal G6PD activity and misses most of the moderate G6PD activity especially in female heterozygotes ( Quantitative Glucose-6-Phosphate Dehydrogenase Activity Several quantitative test kits have been introduced in recent years which are superior to the FST in terms of accuracy and sensitivity