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[DOI] [PubMed] [Google Scholar] Czerwinski M., Gibbs J.P., Slattery J.T
doi: 10.1016/0304-4165(85)90182-5
During denaturing, free reduced Cys residues in control and experimental samples are alkylated using either light ( 12 C 2 H 4 INO) or heavy ( 13 C 2 D 2 H 2 INO) IAM, respectively which results in the addition of a carbamidomethyl group (CAM), adding an extra of 57 or 61 Da to cysteine residues (Figure 4A)
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