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One hour before the assay, the cells were washed twice with FAO assay buffer (Seahorse Bioscience), and 15 min before the assay, the fatty acid oxidation substrate palmitate-BSA or a BSA control (Seahorse Bioscience) was added and the oxygen consumption rate (OCR) with or without palmitate-BSA was measured

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In this article, we will make a short review of LDL metabolism, discuss the discordance between LDL-C and LDL-P, outline the atherogenic mechanisms of action of LDL by focusing on sdLDL and ox-LDL, summarize the methods used for measurement of LDL subclasses, and conclude the advances in LDL-lowering therapies using statins and PCSK9i

doi: 10.1515/CCLM.2000.162

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