glutathione column protocol What is a GST Pull-Down Assay? The Complete Overview with Protocols an – GoldBio Protocol for PurKine™ GST Purification
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Dunsmoor et al., 2019)

Cold water (refrigerated or chilled) slows dissolution and can cause incomplete mixing, leaving undissolved peptide aggregates at the vial bottom
& Kesselheim, A

Our results showed a rather unexpectedly rapid response of the OL lineage cells to the CPZ challenge