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For example, zinc treatment has been shown to attenuate ER stress in porcine oocyte maturation by upregulating ZIP14 and ZIP10 and restoring redox homeostasis, ultimately improving developmental outcomes 121

Access this article Similar content being viewed by others Abbreviations AAPH: Hydrochloride 2,2-azobis-2-amidinopropane AIDS: Acquired immunodeficiency syndrome ARE: Antioxidant response element CAT: Catalase Cu/Zn-SOD: Copper/zinc-superoxide dismutase DIVEMA: Divinyl ether and maleic anhydride DNA: Deoxyribonucleic acid EC-SOD: Extracellular-superoxide dismutase Fe-SOD: Iron-superoxide dismutase FIV: Feline immunodeficiency virus GPx: Glutathione peroxidase H 2 O 2 : Hydrogen peroxide HIV: Human immunodeficiency virus HO: Hydroxyl radical Mn-SOD: Manganese-superoxide dismutase Nrf2: Transcription factor nuclear-factor-E2-related factor O 2 - : Superoxide anion radical PEG: Pegylated PMA: Phorbol 12-myristate 13-acetate ROS: Reactive oxygen species SOD: Superoxide dismutase REFERENCES Davies KJA

Ca-a Cancer J Clin

doi: 10.1016/j.jnutbio.2020.108504 50 ShambergerRJRudolphG

Plasmid construction and transient transfection The ORF of the Notch2 cDNA was amplified by RT-PCR using specific primers (forward, 5-ATG CCC GCC CTG CGC CCC GCT CT-3 and reverse, 5-TTA TAA CTT AAG ACA ATG CCC T-3) that were designed based on the Notch2 gene (GenBank ID: NM_001200001.1) by Takara (Shiga, Japan)