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Stereological and histological studies exhibited that, after busulfan treatment, some large vacuoles were observed in the semi-niferous tubules in the rat testis and the size of seminiferous tubules was reduced, which was linked to disorganization and reduction of height of germinal epithelium following depletion of spermatogenic lineage cells (spermatogonia, spermatocytes, and round elongated spermatid)