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This heterodimer translocates to the nucleus, where HIF-1 binds the activating protein CREB ( P300 ) and the hypoxia reactive element ( HRE ), and subsequently initiates a series of hypoxia-induced cascades, including angiogenesis, through increased transcription of target genes, such as VEGF 28

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This means that even at a LOD of 1 fM and assuming that all 10 5 target molecules reach the detecting surface and bind to a receptor, there is a binding event involving one recognition element for every ten million (10 7 ) populating the detecting interface

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