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The cells were kept at 37C under a humidified 5% CO 2 atmosphere
Samples were spun for 10 min at 4000 g and supernatants were collected and precleared with magnetic protein-G-dynabeads (Invitrogen)
The effects of flaxseed supplementation on metabolic status in women with polycystic ovary syndrome: a randomized open-labeled controlled clinical trial
