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FIGURE 1 2.3 Fetal sex determination and molecular analysis We isolated genomic DNA from the fetal tail using the HotSHOT method (Truett et al., 2000) and determined fetal sex using a PCR-based assay previously described (Thomas et al., 2021)

Although lysosomal pH and cathepsin B activity remained normal, morphological changes after Bafilomycin A1 treatment point to defects in autophagic lysosome reformation (ALR), a cellular process responsible for regenerating functional lysosomes following autophagy, further supporting the role of GDAP1 in PIK fyve -mediated lysosomal maturation

doi: 10.1016/j.tcb.2020.02.009 189 QiXZhaoYQiZHouSChenJ

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