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Western blot Mouse skin was ground into powder under liquid nitrogen, and an appropriate amount of protein lysis solution radioimmunoprecipitation assay and an equal proportion of protease inhibitor PMSF were added

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Once inside the cell, dehydroascorbate is enzymatically reduced to ascorbate, regenerating the active form of vitamin C

To mitigate ethanol accumulation, feeding solutions were added at rates matching the glucose consumption rate, effectively maintaining ethanol levels below 15 g/L while supporting both cell growth and glutathione production