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Analyzing the depolarization kinetics curve can provide crucial clues about the target of action for unknown antibiotics, aiding in novel drug discovery or resistance mechanism studies, and offering direct, visual experimental evidence for synergistic mechanisms in combination therapy, thereby significantly enhancing the platforms potential for mechanistic research and application

A degraded lyophilised vial may appear yellowish rather than white, may fail to dissolve despite adequate water and time, or may produce a yellow-tinted solution

Authors' contributions KN conducted reference research, contributed discussion and figure concerning signalling complexes

It should be noted, however, that these particle size changes are influenced by multiple factors, including cow breed, feed, and season 99