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Fruiting body polysaccharides of Hericium erinaceus induce apoptosis in human colorectal cancer cells via ROS generation mediating caspase-9-dependent signaling pathways

Timelapse imaging revealed no significant changes in the 405/488 fluorescence ratio in the nucleoli during the cell cycle ( Figure 3d )

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After final washing, proteinantibody complexes were visualized using enhanced chemiluminescence (ECL) substrate (Super Signal West Femto, Thermo Fisher Scientific) and imaged with an Azure Biosystems c500 imaging system