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RIPA buffer (50 m M Tris pH 8, 150 m M NaCl, 1% NP-40, 0.5% deoxycholic acid, and 0.1% SDS) was supplemented with protease and phosphatase inhibitors (1 m M phenylmethylsulfonyl fluoride, 5 g/mL leupeptin, 2 g/mL aprotinin, 1 m M EDTA, 10 m M NaF, and 1 m M NaVO4)

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