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Hydrogen also exerts a protective effect on intestinal cells by inhibiting the expression of related inflammatory pathways 29 and reducing downstream inflammatory factors

Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice

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[1] Persistently high B12 levels warrant medical investigation, as they may signal liver disease, kidney dysfunction, or certain haematological disorders