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Detection of Antioxidant Enzyme Activities Ascorbate peroxidase activity was detected as described in Dehydroascorbate reductase (DHAR) activity was determined as described in Glutathione reductase activity was detected as described in Preparation of Nonprotein Thiol Extracts The alga (0.2 g of DT) was frozen in liquid nitrogen and homogenized in a mortar with a pestle and extracted by addition of 1.2 ml of 0.1% (w/v) trifluoroacetic acid (TFA)6.3 mM diethylenetriaminepentaacetic acid (DTPA)

A final volume of 80 L was frozen and stored at 80 C until required for sequencing

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