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Description
Cells were incubated with 0.125 M glycine to terminate cross-linking, washed twice with PBS and lysed with SDS nuclear lysis buffer (1% SDS, 10 mM EDTA and 50 mM Tris-HCl, pH 8.1) for 10 min on ice

A mild tingling is normal

Sections were stained with hematoxylin and eosin (H&E) for general morphology

doi: 10.1097/00001721-199910000-00006 [DOI] [PubMed] [Google Scholar] 350

Banerjee A, Qi J, Gogoi R, Wong J, Mitragotri S