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Purified sgRNA (50 ng/L) was microinjected into the pronucleus of fertilized embryos derived from Rosa26-Cas9 knock-in female mice (The Jackson Laboratory)

It also inhibits glucagon secretion, preventing excess glucose production by the liver [6]

Khairallah RJ, Sparagna GC, Khanna N, OShea KM, Hecker PA, Kristian T, Fiskum G, Des Rosiers C, Polster BM, Stanley WC
