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The principle is straightforward: a purified bait protein is immobilized on an affinity matrixtypically through a fusion tag or high-specificity ligandand then incubated with a protein mixture containing potential binding partners (prey)

BioMed Res Int 2021:2648065 Luo J, Nikolaev AY, Imai S, Chen D, Su F, Shiloh A, Guarente L, Gu W (2001) Negative control of p53 by Sir2_ promotes cell survival under stress

It was derived from epithalamin, a polypeptide complex naturally secreted by the pineal gland in very small quantities

Footnotes Conflict of interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest

reuteri produces tryptophan metabolites, including indole derivatives, that activate AhR, leading to Foxp3 expression and IL-10 production to suppress Th17 cells (104, 137, 138)