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Another enzyme is AHCY, which converts S-adenosylhomocysteine (SAH) to homocysteine and interacts with the CLOCK-BMAL1 complex, playing a key role in circadian regulation [35]

In several preclinical studies, ASX has been shown to have an indirect antioxidant effect by activating the transcription factor nuclear factor erythroid 2-related factor 2 and increasing the expression of its antioxidant target genes, including phase II biotransformation enzymes [69,70,71,72,73,74]

Drug Des Devel Ther (2020) 14:490113

In the ATP-bound conformation, the mutation of Gly to Asp will have limited expected effects, resulting most probably in a rotamer rearrangement in respect of D999 with limited consequences on the backbone position