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Cell debris was removed by centrifuging for 20 min at 20,000 g at 4 C in a pre-cooled table centrifuge

3.3.2 4-hydroxy-2-nonenal 4-hydroxy-2-nonenal (4-HNE) is a lipid peroxide produced by polyunsaturated fatty acids (PUFAs) in response to oxidative stress (Figure 3)

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Recent developments in research techniques, such as single-cell RNA-sequencing, have accurately dissected gene expression changes following NMDAR-induced injuries in the mouse retina, clarifying the role of microglia in regulating inflammatory cytokines [60]

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