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doi: 10.3390/antiox11040795, PMID: [DOI] [PMC free article] [PubMed] [Google Scholar] 117.Young AJ, Lowe GM

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Breastfeeding typically requires an additional 300 to 500 calories a day, according to Mayo Clinic, to support milk production and energy

Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)

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