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This methanol is produced by the hydrolysis of methyl esters in pectins as catalyzed by pectin methylesterases from gut bacteria (PME, EC 3.1.1.11, Figure 7E

Stability data measured by monomer retention over six months at 50 C showed that formulations with higher trehalose or sucrose content were more stable

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Then, siRNA/Ap-CS nanostructure was constructed as described in Methods, and its stepwise assembly was characterized by dynamic light scattering (DLS) where intermediate formulations serve as controls

The freeze-thaw cycle can damage the peptide's structure through the formation of ice crystals, leading to aggregation and loss of bioactivity