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Rabbit anti-Akt1 (Invitrogen, #PA5-29169, 1:100) and mouse anti-mCherry (Abcam, #ab125096, 1:500) were used as primary antibodies, and Alexa Fluor 488-conjugated anti-rabbit (Invitrogen, #A32731, 1:400) and Alexa Fluor 555-conjugated anti-mouse (Invitrogen, #A32727, 1:400) were used as secondary antibodies